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Publications


2021


Schweitzer F, Goereci Y, Franke C, Silling S, Bösl F, Maier F, Heger E, Deiman B, Prüss H, Onur OA, Klein F, Fink G, Di Cristanziano V, Warnke C. CSF analysis in post-COVID-19 is not suggestive of persistent CNS infection.
Ann Neurol. (2021)
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Müller L, Andrée M, Ostermann PN, Jazmati N, Flüh G, Fischer JC, Bölke E, Heger E, Vanshylla K, Klein F, Wisplinghoff H, Schaal H, Drexler I, Walker A, Adams O, Timm J. SARS-CoV-2 Infection in Fully Vaccinated Individuals of Old Age Strongly Boosts the Humoral Immune Response.
Front Med (Lausanne). (2021)
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Tober-Lau P, Schwarz T, Vanshylla K, Hillus D, Gruell H; EICOV/COVIM Study Group, Suttorp N, Landgraf I, Kappert K, Seybold J, Drosten C, Klein F, Kurth F, Sander LE, Corman VM. Long-term immunogenicity of BNT162b2 vaccination in older people and younger health-care workers.
Lancet Respir Med. (2021)
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Stemler J, Kramer T, Dimitriou V, Wieland U, Schumacher S, Sprute R, Oberste M, Wiesmüller G, Rau H, Pieper S, Bethe U, Lehmann C, Hellmich M, Klein F, Langebartels G, Cornely OA. Mobile PCR-based surveillance for SARS-CoV-2 to reduce visiting restrictions in nursing homes during the COVID-19 pandemic: a pilot study.
Infection. (2021)
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Joachim A, Dewald F, Suárez I, Zemlin M, Lang I, Stutz R, Marthaler A, Bosse HM, Lübke N, Münch J, Bernard MA, Jeltsch K, Tönshoff B, Weidner N, Kräusslich HG, Birzele L, Hübner J, Schmied P, Meyer-Bühn M, Horemheb-Rubio G, Cornely OA, Haverkamp H, Wiesmüller G, Fätkenheuer G, Hero B, Kaiser R, Dötsch J, Rybniker J; B-FAST study group. Pooled RT-qPCR testing for SARS-CoV-2 surveillance in schools - a cluster randomised trial.
EClinicalMedicine. (2021)
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Meyer M, Holfter A, Ruebsteck E, Gruell H, Dewald F, Koerner RW, Klein F, Lehmann C, Huenseler C, Weber LT. The Alpha Variant (B.1.1.7) of SARS-CoV-2 in Children: First Experience from 3544 Nucleic Acid Amplification Tests in a Cohort of Children in Germany.
Viruses. (2021)
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Halwe S, Kupke A, Vanshylla K, Liberta F, Gruell H, Zehner M, Rohde C, Krähling V, Gellhorn Serra M, Kreer C, Klüver M, Sauerhering L, Schmidt J, Cai Z, Han F, Young D, Yang G, Widera M, Koch M, Werner A, Kämper L, Becker N, Marlow MS, Eickmann M, Ciesek S, Schiele F, Klein F, Becker S. Intranasal Administration of a Monoclonal Neutralizing Antibody Protects Mice against SARS-CoV-2 Infection.
Viruses. (2021)
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Meijers M, Vanshylla K, Gruell H, Klein F, Lässig M. Predicting in vivo escape dynamics of HIV-1 from a broadly neutralizing antibody.
Proc Natl Acad Sci U S A. (2021)
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Oberste M, Pusch LM, Roth R, Shah-Hosseini K, Dewald F, Müller C, Stach von Goltzheim L, Lehmann C, Buess M, Wolff A, Fätkenheuer G, Wiesmüller G, Klein F, Hellmich M, Neuhann F. Protocol of the Cologne Corona Surveillance (CoCoS) Study- a prospective population-based cohort study.
BMC Public Health. (2021)
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Korenkov M, Poopalasingam N, Madler M, Vanshylla K, Eggeling R, Wirtz M, Fish I, Dewald F, Gieselmann L, Lehmann C, Fätkenheuer G, Gruell H, Pfeifer N, Heger E, Klein F. Evaluation of a Rapid Antigen Test To Detect SARS-CoV-2 Infection and Identify Potentially Infectious Individuals.
J Clin Microbiol. (2021)
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Reif T, Dyckhoff G, Hohenberger R, Kolbe CC, Gruell H, Klein F, Latz E, Stolp B, Fackler OT. Contact-dependent inhibition of HIV-1 replication in ex vivo human tonsil cultures by polymorphonuclear neutrophils.
Cell Rep Med. (2021)
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Wennhold K, Thelen M, Lehmann J, Schran S, Preugszat E, Garcia-Marquez M, Lechner A, Shimabukuro-Vornhagen A, Ercanoglu MS, Klein F, Thangarajah F, Eidt S, Löser H, Bruns C, Quaas A, von Bergwelt-Baildon M, Schlößer HA. CD86+ Antigen-Presenting B Cells Are Increased in Cancer, Localize in Tertiary Lymphoid Structures, and Induce Specific T-cell Responses.
Cancer Immunol Res. (2021)
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Theobald SJ, Simonis A, Georgomanolis T, Kreer C, Zehner M, Eisfeld HS, Albert MC, Chhen J, Motameny S, Erger F, Fischer J, Malin JJ, Gräb J, Winter S, Pouikli A, David F, Böll B, Koehler P, Vanshylla K, Gruell H, Suárez I, Hallek M, Fätkenheuer G, Jung N, Cornely OA, Lehmann C, Tessarz P, Altmüller J, Nürnberg P, Kashkar H, Klein F, Koch M, Rybniker J. Long-lived macrophage reprogramming drives spike protein-mediated inflammasome activation in COVID-19.
EMBO Mol Med. (2021)
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Gieselmann L, Kreer C, Ercanoglu MS, Lehnen N, Zehner M, Schommers P, Potthoff J, Gruell H, Klein F. Effective high-throughput isolation of fully human antibodies targeting infectious pathogens.
Nature Protocols (2021)
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Augustin M, Schommers P, Stecher M, Dewald F, Gieselmann L, Gruell H, Horn C, Vanshylla K, Cristanziano VD, Osebold L, Roventa M, Riaz T, Tschernoster N, Altmueller J, Rose L, Salomon S, Priesner V, Luers JC, Albus C, Rosenkranz S, Gathof B, Fätkenheuer G, Hallek M, Klein F, Suárez I, Lehmann C. Post-COVID syndrome in non-hospitalised patients with COVID-19: a longitudinal prospective cohort study.
Lancet Reg Health (2021)
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Vanshylla K, Di Cristanziano V, Kleipass F, Dewald F, Schommers P, Gieselmann L, Gruell H, Schlotz M, Ercanoglu MS, Stumpf R, Mayer P, Zehner M, Heger E, Johannis W, Horn C, Suárez I, Jung N, Salomon S, Eberhardt KA, Gathof B, Fätkenheuer G, Pfeifer N, Eggeling R, Augustin M, Lehmann C, Klein F. Kinetics and correlates of the neutralizing antibody response to SARS-CoV-2 infection in humans.
Cell Host Microbe (2021)
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Meyer M, Ruebsteck E, Gruell H, Klein F, Lehmann C, Wendt S, Huenseler C, Weber LT. COVID-19 study found that 0.4% of 5730 asymptomatic children aged 0-18 years tested positive for virus before hospital procedures or admission.
Acta Paediatr. (2021)
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Bamidis AD, Koehler P, di Cristanziano V, Rasche K, Demirel B, Bacher P, Hallek M, Kochanek M, Klein F, Hofmann SC, Wesselmann U, Kofler DM. First manifestation of adult-onset Still's disease after COVID-19.
Lancet Rheumatol. (2021)
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Eberhardt KA, Dewald F, Heger E, Gieselmann L, Vanshylla K, Wirtz M, Kleipass F, Johannis W, Schommers P, Gruell H, Brensing KA, Müller RU, Augustin M, Lehmann C, Koch M, Klein F, Di Cristanziano V. Evaluation of a New Spike (S)-Protein-Based Commercial Immunoassay for the Detection of Anti-SARS-CoV-2 IgG.
Microorganism (2021)
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Vanshylla K, Held K, Eser TM, Gruell H, Kleipass F, Stumpf R, Jain K, Weiland D, Münch J, Grüttner B, Geldmacher C, Klein F. CD34T+ Humanized Mouse Model to Study Mucosal HIV-1 Transmission and Prevention.
Vaccines (2021)
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Theobald SJ, Kreer C, Khailaie S, Bonifacius A, Eiz-Vesper B, Figueiredo C, Mach M, Backovic M, Ballmaier M, Koenig J, Olbrich H, Schneider A, Volk V, Danisch S, Gieselmann L, Ercanoglu MS, Messerle M, von Kaisenberg C, Witte T, Klawonn F, Meyer-Hermann M, Klein F, Stripecke R. Correction: Repertoire characterization and validation of gB-specific human IgGs directly cloned from humanized mice vaccinated with dendritic cells and protected against HCMV.
PLoS Pathog. (2021)
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Di Cristanziano V, Meyer-Schwickerath C, Eberhardt KA, Rybniker J, Heger E, Knops E, Hallek M, Klein F, Holtick U, Jung N. Detection of SARS-CoV-2 viremia before onset of COVID-19 symptoms in an allo-transplanted patient with acute leukemia.
Bone Marrow Transplant (2021)
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Müller L, Ostermann PN, Walker A, Wienemann T, Mertens A, Adams O, Andree M, Hauka S, Lübke N, Keitel V, Drexler I, Di Cristanziano V, Hermsen DF, Kaiser R, Boege F, Klein F, Schaal H, Timm J, Senff T. Sensitivity of anti-SARS-CoV-2 serological assays in a high-prevalence setting.
Eur J Clin Microbiol Infect Dis. (2021)
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Rokohl AC, Grajewski RS, Wawer Matos PA, Koch HL, Dewald F, Klein F, Fätkenheuer G, Lehmann C, Cursiefen C, Heindl LM. No secret hiding place? Absence of SARS-CoV-2 on the ocular surface of 1145 hospitalized patients in a pandemic area.
Graefes Arch Clin Exp Ophtalmol. (2021)
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Horn C, Augustin M, Ercanoglu MS, Heger E, Knops E, Bondet V, Duffy D, Chon SH, Nierhoff D, Oette M, Schäfer H, Vivaldi C, Held K, Anderson J, Geldmacher C, Suárez I, Rybniker J, Klein F, Fätkenheuer G, Müller-Trutwin M, Lehmann C. HIV DNA reservoir and elevated PD-1 expression of CD4 T-cell subsets particularly persist in the terminal ileum of HIV-positive patients despite cART.
HIV Med. (2021)
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Krähling V, Halwe S, Rohde C, Becker D, Berghöfer S, Dahlke C, Eickmann M, Ercanoglu MS, Gieselmann L, Herwig A, Kupke A, Müller H, Neubauer-Rädel P, Klein F, Keller C, Becker S. Development and characterization of an indirect ELISA to detect SARS-CoV-2 spike protein-specific antibodies.
J Immunol Methods (2021)
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Bankwitz D, Bahai A, Labuhn M, Doepke M, Ginkel C, Khera T, Todt D, Ströh LJ, Dold L, Klein F, Klawonn F, Krey T, Behrendt P, Cornberg M, McHardy AC, Pietschmann T. Hepatitic C reference virses highlight potent antibody responses and diverse viral functional interactions with neutralising antibodies.
Gut (2020)
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2020


Kalusche S, Vanshylla K, Kleipass F, Gruell H, Müller B, Zeng Z, Koch K, Stein S, Marcotte H, Klein F, Dietrich U. Lactobacilli Expresing Broadly Neutralizing Nanobodies against HIV-1 as Potential Vectors fpr HIV-1 Prohylaxis?
Vaccines (2020)
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Kaw S, Ananth S, Tsopoulidis N, Morath K, Coban BM, Hohenberger R, Bulut OC, Klein F, Stolp B, Fachler OT. Expression of HIV-1 pathogenesis factor NEF in CD4 T cells impairs antigen-specific B-cell function.
EMBO J. (2020)
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Schermer B, Fabretti F, Damagenz M, Di Cristanziano V, Heger E, Arjune S, Tanner NA, Imhof T, Koch M, Ladha A, Joung J, Gootenberg JS, Abudayyeh OO, Burst V, Zhang F, Klein F, Benzing T, Müller RU. Rapid SARS-CoV-2 testing in primary material based on a novel multiplex RT-LAMP assay.
PLoS One (2020)
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Eberhardt KA, Meyer-Schwickerath C, Heger E, Knops E, Lehmann C, Rybniker J, Schommers P, Eichenauer DA, Kurth F, Ramharter M, Kaiser R, Holtick U, Klein F, Jung N, Di Cristanziano V. RNAemia Corresponds to Disease Severity and Antibody Response in Hospitalized COVID-19 Patients.
Viruses (2020)
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Di Cristanziano V, Meyer-Schwickerath C, Eberhardt KA, Rybniker J, Heger E, Knops E, Hallek M, Klein F, Holtick U, Jung N. Detection of SARS-CoV-2 viremia before onset of COVID-19 symptoms in an allo-transplaned patient with acute leukemia.
Bone Marrow Transplant. (2020)
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Ramani A, Müller L, Ostermann PN*, Gabriel E, Abida-Islam P, Müller-Schiffmann A, Mariappan A, Goureau O, Gruell H, Walker A, Andrée M, Hauka S, Houwaart T, Dilthey A, Wohlgemuth K, Omran H, Klein F, Wieczorek D, Adams O, Timm J, Korth C, Schaal H, Gopalakrishnan J. SARS-CoV-2 targets neurons of 3D human brain organoids.
EMBO J. (2020)
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Rokohl AC, Loreck N, Wawer Matos PA, Zwingelberg S, Augustin M, Dewald F, Grajewski RS, Klein F, Lehmann C, Heindl LM. More than loss of taste and smell: burning watering eyes in coronavirus disease 2019.
Clin Microbiol Infect. (2020)
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Kreer C, Zehner M, Weber T, Ercanoglu MS, Gieselmann L, Rohde C, Halwe S, Korenkov M, Schommers P, Vanshylla K, Di Cristanziano V, Janicki H, Brinker R, Ashurov A, Krähling V, Kupke A, Cohen-Dvashi H, Koch M, Eckert JM, Lederer S, Pfeifer S, Wolf T, Vehreschild MJGT, Wendtner C, Diskin R, Gruell H, Becker S, Klein F. Longitudinal isolation of potent near-germline SARS-CoV-2-neutralizing antibodies from COVID-19 patients.
Cell (2020)
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Stripecke R, Münz C, Schuringa JJ, Bissig KD, Soper B, Meeham T, Yao LC, Di Santo JP, Brehm M, Rodriguez E, Wege AK, Bonnet D, Guionaud S, Howard KE, Kitchen S, Klein F, Saeb-Parsy K, Sam J, Sharma AD, Trumpp A, Trusolino L, Bult C, Shultz L. Innovations, challenges, and minimal information for standardization of humanized mice.
EMBO Mol Med. (2020)
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Langerbeins P, Fürstenau M, Gruell H, Klein F, Persigehl T, Rybniker J, Seeger-Nukpezah T, Kochanek M, Hallek M, Eichhorst B, Koehler P, Böll B. COVID-19 Complicated by Parainfluenza Co-Infection in a Patient With Chronic Lymphocytic Leukemia.
Eur J Haematol. (2020)
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Grajewski RS, Rokohl AC, Becker M, Dewald F, Lehmann C, Fätkenheuer G, Cursiefen C, Klein F, Heindl LM. A missing link between SARS-CoV-2 and the eye?: ACE2 expression on the ocular surface.
J Med Virol. (2020)
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Augustin M, Schommers P, Suárez I, Koehler P, Gruell H, Klein F, Maurer C, Langerbeins P, Priesner V, Schmidt-Hellerau K, Malin JJ, Stecher M, Jung N, Wiesmüller G, Meissner A, Zweigner J, Langebartels G, Kolibay F, Suárez V, Burst V, Valentin P, Schedler D, Cornely OA, Hallek M, Fätkenheuer G, Rybniker J, Lehmann C. Rapid Response Infrastructure for Pandemic Preparedness in a Tertiary Care Hospital: Lessons Learned From the COVID-19 Outbreak in Cologne, Germany, February to March 2020
Euro Surveill.
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Luers JC, Rokohl AC, Loreck N, Wawer Matos PA, Augustin M, Dewald F, Klein F, Lehmann C, Heindl LM. Reply to Gourtsoyannis.
Clin Infect Dis. (2020)
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Heidepriem J, Krähling V, Dahlke C, Wolf T, Klein F, Addo MM, Becker S, Loeffler FF. Epitopes of Naturally Acquired and Vaccine-Induced Anti-Ebola Virus Glycoprotein Antibodies in Single Amino Acid Resolution
Biotechnol J. (2020)
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Luers JC, Rokohl AC, Loreck N, Wawer Matos PA, Augustin M, Dewald F, Klein F, Lehmann C, Heindl LM. Olfactory and Gustatory Dysfunction in Coronavirus Disease 19 (COVID-19).
Clin Infect Dis. (2020)
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Koehler P, Cornely OA, Böttiger BW, Dusse F, Eichenauer DA, Fuchs F, Hallek M, Jung N, Klein F, Persigehl T, Rybniker J, Kochanek M, Böll B, Shimabukuro-Vornhagen A. COVID-19 associated pulmonary aspergillosis.
Mycoses (2020)
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Zehner M and Klein F. Antibody Teamwork against Ebola Virus Disease.
Immunity (2020)
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Cohen-Dvashi H, Zehner M, Ehrhardt S, Katz M, Elad N, Klein F, Diskin R. Structural Basis for a Convergent Immune Response against Ebola Virus.
Cell Host Microbe (2020)
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Niessl J, Baxter AE, Mendoza P, Jankovic M, Cohen YZ, Butler AL, Lu CL, Dubé M, Shimeliovich I, Gruell H, Klein F, Caskey M, Nussenzweig MC, Kaufmann DE. Combination anti-HIV-1 antibody therapy is associated with increased virus-specific T cell immunity.
Nat Med (2020)
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Schommers P, Gruell H, Abernathy ME, Tran M-K, Dingens AS, Gristick HB, Barnes CO, Schoofs T, Schlotz M, Vanshylla K, Kreer C, Weiland D, Holtick U, Scheid C, Valter MV, van Gils MJ, Sanders RW, Vehreschild JJ, Cornely OA, Lehmann C, Fätkenheuer G, Seaman MS, Bloom JD, Bjorkman J, Klein F. Restriction of HIV-1 Escape by a Highly Broad and Potent Neutralizing Antibody.
Cell (2020)
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Kreer C, Döring M, Lehnen N, Ercanoglu MS, Gieselmann L, Luca D, Jain K, Schommers P, Pfeifer N, Klein F. OpenPrimeR for multiplex amplification of highly diverse templates.
J Immunol Methods (2020)
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Kreer C, Gruell H, Mora T, Walczak A.M. and Klein F. Exploiting B Cell Receptor Analyses to Inform on HIV-1 Vaccination Strategies.
Vaccines (2020)
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2019


Ehrhardt SA*, Zehner M*, Krähling V, Cohen-Dvashi H, Kreer C, Elad N, Gruell H, Ercanoglu MS, Schommers P, Gieselmann L, Eggeling R, Dahlke C, Wolf T, Pfeifer N, Addo MM, Diskin R, Becker S, Klein F. Polyclonal and convergent antibody response to Ebola virus vaccine rVSV-ZEBOV.
Nat Med (2019)
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Döring M, Kreer C, Lehnen N, Klein F, Pfeifer N. Modeling the Amplification of Immunoglobulins through Machine Learning on Sequence-Specific Features.
Sci Rep. (2019)
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Schleimann MH, Kobberø ML, Vibholm LK, Kjær K, Giron LB, Busman-Sahay K, Chan CN, Nekorchuk M, Schmidt M, Wittig B, Damsgaard TE, Ahlburg P, Hellfritzsch MB, Zuwala K, Rothemejer FH, Olesen R, Schommers P, Klein F, Dweep H, Kossenkov A, Nyengaard JR, Estes JD, Abdel-Mohsen M, Østergaard L, Tolstrup M, Søgaard OS, Denton PW. TLR9 agonist MGN1703 enhances B cell differentiation and function in lymph nodes.
EBioMedicine. (2019)
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Schoofs T, Barnes CO, Suh-Toma N, Golijanin J, Schommers P, Gruell H, West AP Jr, Bach F, Lee YE, Nogueira L, Georgiev IS, Bailer RT, Czartoski J, Mascola JR, Seaman MS, McElrath MJ, Doria-Rose NA, Klein F, Nussenzweig MC, Bjorkman PJ. Broad and Potent Neutralizing Antibodies Recognize the Silent Face of the HIV Envelope.
Immunity. (2019)
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Stecher M, Claßen A, Klein F, Lehmann C, Gruell H, Platten M, Wyen C, Behrens G, Fätkenheuer G, Vehreschild JJ. Systematic Review And Meta-Analysis of Treatment Interruptions in HIV-1 Infected Patients Receiving Antiretroviral Therapy: Implications for Future HIV Cure Trials.
Clin Infect Dis.. (2019)
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Caskey M, Klein F, Nussenzweig MC. Broadly neutralizing anti-HIV-1 monoclonal antibodies in the clinic.
Nat Med. (2019)
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2018


Gruell H and Klein F. Antibody-mediated prevention and treatment of HIV-1 infection.
Retrovirology. (2018)
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Khera T, Behrendt P, Bankwitz D, Brown RJP, Todt D, Doepke M, Ghafoor Khan A, Schulze K, Law J, Logan M, Hockman D, Wong JAJ, Dold L, Gonzalez-Motos V, Spengler U, Viejo-Borbolla A, Ströh L, Krey T, Tarr AW, Steinmann E, Manns MP, Klein F, Guzman CA, Marcotrigiano J, Houghton M, Pietschmann T. Functional and immunogenic characterization of diverse HCV glycoprotein E2 variants.
J Hepatol. (2018)
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Lu CL, Pai JA, Nogueira L, Mendoza P, Gruell H, Oliveira TY, Barton J, Lorenzi JCC, Cohen YZ, Cohn LB, Klein F, Caskey M, Nussenzweig MC, Jankovic M. Relationship between intact HIV-1 proviruses in circulating CD4+ T cells and rebound viruses emerging during treatment interruption.
PNAS. (2018)
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Bar-On Y*, Gruell H*, Schoofs T, Pai JA, Nogueira L, Butler AL, Millard K, Lehmann C, Suárez I, Oliveira TY, Karagounis T, Cohen YZ, Wyen C, Scholten S, Handl L, Belblidia S, Dizon JP, Vehreschild JJ, Witmer-Pack M, Shimeliovich I, Jain K, Fiddike K, Seaton KE, Yates NL, Horowitz J, Gulick RM, Pfeifer N, Tomaras GD, Seaman MS, Fätkenheuer G, Caskey M*, Klein F*, Nussenzweig MC*. Safety and antiviral activity of combination HIV-1 broadly neutralizing antibodies in viremic individuals.
Nat Med. (2018)
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Mendoza P*, Gruell H*, Nogueira L, Pai JA, Butler AL, Millard K, Lehmann C, Suárez I, Oliveira TY, Lorenzi JCC, Cohen YZ, Wyen C, Kümmerle T, Karagounis T, Lu CL, Handl L, Unson-O'Brien C, Patel R, Ruping C, Schlotz M, Witmer-Pack M, Shimeliovich I, Kremer G, Thomas E, Seaton KE, Horowitz J, West AP Jr, Bjorkman PJ, Tomaras GD, Gulick RM, Pfeifer N, Fätkenheuer G, Seaman MS, Klein F*, Caskey M*, Nussenzweig MC*. Combination therapy with anti-HIV-1 antibodies maintains viral suppression.
Nature. (2018)
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Meder L, König K, Dietlein F, Macheleidt I, Florin A, Ercanoglu MS, Rommerscheidt-Fuss U, Koker M, Schön G, Odenthal M, Klein F, Büttner R, Schulte JH, Heukamp LC, Ullrich RT., LIN28B enhanced tumorigenesis in an autochthonous KRASG12V-driven lung carcinoma mouse model.
Oncogene. (2018)
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2017


Nishimura Y., Gautam R., Chun T.W., Sadjadpour R., Foulds K.E., Shingai M., Klein F., Gazumyan A., Golijanin J., Donaldson M., Donau O.K., Plishka R.J., Buckler-White A., Seaman M.S., Lifson J.D., Koup R.A., Fauci A.S., Nussenzweig M.C., Martin M.A., Early antibody therapy can induce long-lasting immunity to SHIV.
Nature. (2017). read more


Gruell H. and Klein F., Progress in HIV-1 antibody research using humanized mice.
Curr Opin HIV AIDS. (2017). readmore


Caskey M.*, Schoofs T.*, Gruell H.*, Settler A., Karagounis T., Kreider E.F., Murrell B., Pfeifer N., Nogueira L., Oliveira T.Y., Learn G.H., Cohen Y.Z., Lehmann C., Gillor D., Shimeliovich I., Unson-O’Brien C., Weiland D., Robles A., Kümmerle T., Wyen C., Levin R., Witmer-Pack M., Eren K., Ignacio C., Kiss S., West A.P., Mouquet H., Zingman B.S., Gulick R.M., Keler T., Bjorkman P.J., Seaman M.S., Hahn B.H., Fätkenheuer G., Schlesinger S.J., Nussenzweig M.C.*, Klein F.*, Antibody 10-1074 suppresses viremia in HIV-1-infected individuals.
Nat Med. (2017). read more


2016


Caskey, M., Klein, F., Nussenzweig, M.C., Broadly Neutralizing Antibodies for HIV-1 Prevention or Immunotherapy.
N Engl J Med. (2016). read more


Gristick, H.B., von Boehmer, L., West, A.P. Jr, Schamber, M., Gazumyan, A., Golijanin, J., Seaman, M.S., Fätkenheuer, G., Klein, F., Nussenzweig, M.C., Bjorkman, P.J.,. Natively glycosylated HIV-1 Env structure reveals new mode for antibody recognition of the CD4- binding site.
Nat Struct Mol Biol. (2016). read more


Scheid, J.F.*, Horwitz, J.A.*, Bar-On, Y., Kreider, E.F., Lu, C.L., Lorenzi, J.C., Feldmann, A., Braunschweig, M., Nogueira, L., Oliveira, T., Shimeliovich, I., Patel, R., Burke, L., Cohen, Y.Z., Hadrigan, S., Settler, A., Witmer-Pack, M., West, A.P. Jr, Juelg, B., Keler, T., Hawthorne, T., Zingman, B., Gulick, R.M., Pfeifer, N., Learn, G.H., Seaman, M.S., Bjorkman, P.J., Klein, F., Schlesinger, S.J., Walker, B.D., Hahn, B.H., Nussenzweig, M.C., HIV-1 antibody 3BNC117 suppresses viral rebound in humans during treatment interruption.
Nature. (2016). read more


Schoofs, T.*, Klein, F.*, Braunschweig, M.*, Kreider, E.F., Feldmann, A., Nogueira, L., Oliveira, T., Lorenzi, J.C., Parrish, E.H., Learn, G.H., West, A.P. Jr, Bjorkman, P.J., Schlesinger, S.J., Seaman, M.S., Czartoski, J., McElrath, M.J., Pfeifer, N., Hahn, B.H., Caskey, M., Nussenzweig, M.C., HIV-1 therapy with monoclonal antibody 3BNC117 elicits host immune responses against HIV-1.
Science. (2016). read more


Gautam, R., Nishimura, Y., Pegu, A., Nason, M.C., Klein, F., Gazumyan, A., Golijanin, J., Buckler-White, A., Sadjadpour, R., Wang, K., Mankoff, Z., Schmidt, S.D., Lifson, J.D., Mascola, J.R., Nussenzweig, M.C., Martin, M.A., A single injection of anti-HIV-1 antibodies protects against repeated SHIV challenges.
Nature. (2016). read more


Akkina, R.K., Allam, A., Balazs, A.B., Blankson, J., Burnett, J.C., Casares, S., Garcia, J.V., Hasenkrug, K., Kashanchi, F., Kitchen, S., Klein, F., Kumar, P., Luster, A.D., Poluektova, L.Y., Rao, M., Sanders, B.E., Shultz, L.D., and JA, Z., Improvements and Limitations of Humanized Mouse Models for HIV Research: NIH/NIAID "Meet the Experts" 2015 Workshop Summary.
AIDS Res Hum Retroviruses. (2016). read more


2015


Lee, J.H., Leaman, D.P., Kim, A.S., Torrents de la Pena, A., Sliepen, K., Yasmeen, A., Derking, R., Ramos, A., de Taeye, S.W., Ozorowski, G., Klein, F., Burton, D.R., Nussenzweig, M.C., Poignard, P., Moore, J.P., Klasse, P.J., Sanders, R.W., Zwick, M.B., Wilson, I.A., and Ward, A.B., Antibodies to a conformational epitope on gp41 neutralize HIV-1 by destabilizing the Env spike.
Nat Commun (2015). read more


Cohn, L.B., Silva, I.T., Oliveira, T.Y., Rosales, R.A., Parrish, E.H., Learn, G.H., Hahn, B.H., Czartoski, J.L., McElrath, M.J., Lehmann, C., Klein, F., Caskey, M., Walker, B.D., Siliciano, J.D., Siliciano, R.F., Jankovic, M., and Nussenzweig, M.C., HIV-1 integration landscape during latent and active infection.
Cell. (2015). read more


Gruell, H. and Klein, F., Mögliche Strategien zur HIV-Prävention und -Therapie.
MMW Fortschr Med. (2015).


Freund, N.T., Horwitz, J.A., Nogueira, L., Sievers, S.A., Scharf, L., Scheid, J.F., Gazumyan, A., Liu, C., Velinzon, K., Goldenthal, A., Sanders, R.W., Moore, J.P., Bjorkman, P.J., Seaman, M.S., Walker, B.D., Klein, F., and Nussenzweig, M.C., A New Glycan-Dependent CD4-Binding Site Neutralizing Antibody Exerts Pressure on HIV-1 In Vivo.
PLoS Pathog. (2015). read more


Caskey, M.*, Klein, F.*, Lorenzi, J.C., Seaman, M.S., West, A.P., Jr., Buckley, N., Kremer, G., Nogueira, L., Braunschweig, M., Scheid, J.F., Horwitz, J.A., Shimeliovich, I., Ben-Avraham, S., Witmer-Pack, M., Platten, M., Lehmann, C., Burke, L.A., Hawthorne, T., Gorelick, R.J., Walker, B.D., Keler, T., Gulick, R.M., Fatkenheuer, G., Schlesinger, S.J., and Nussenzweig, M.C., Viraemia suppressed in HIV-1-infected humans by broadly neutralizing antibody 3BNC117.
Nature. (2015). read more


Zolla-Pazner, S., Cohen, S.S., Boyd, D., Kong, X.P., Seaman, M., Nussenzweig, M., Klein, F., Overbaugh, J., and Totrov, M., Structure/Function Studies Involving the V3 Region of the HIV-1 Envelope Delineate Multiple Factors that Affect Neutralization Sensitivity.
J Virol. (2015). read more


2014


Bournazos, S., Klein, F., Pietzsch, J., Seaman, M.S., Nussenzweig, M.C., and Ravetch, J.V., Broadly neutralizing anti-HIV-1 antibodies require Fc effector functions for in vivo activity.
Cell. (2014). read more


Halper-Stromberg, A., Lu, C.L., Klein, F., Horwitz, J.A., Bournazos, S., Nogueira, L., Eisenreich, T.R., Liu, C., Gazumyan, A., Schaefer, U., Furze, R.C., Seaman, M.S., Prinjha, R., Tarakhovsky, A., Ravetch, J.V., and Nussenzweig, M.C., Broadly neutralizing antibodies and viral inducers decrease rebound from HIV-1 latent reservoirs in humanized mice.
Cell. (2014). read more


Klein, F., Nogueira, L., Nishimura, Y., Phad, G., West, A.P., Jr., Halper-Stromberg, A., Horwitz, J.A., Gazumyan, A., Liu, C., Eisenreich, T.R., Lehmann, C., Fatkenheuer, G., Williams, C., Shingai, M., Martin, M.A., Bjorkman, P.J., Seaman, M.S., Zolla-Pazner, S., Karlsson Hedestam, G.B., and Nussenzweig, M.C., Enhanced HIV-1 immunotherapy by commonly arising antibodies that target virus escape variants.
J Exp Med. (2014). read more


Gruell, H. and Klein, F., Opening Fronts in HIV Vaccine Development: Tracking the development of broadly neutralizing antibodies.
Nat Med. (2014). read more


West, A.P., Jr., Scharf, L., Scheid, J.F., Klein, F., Bjorkman, P.J., and Nussenzweig, M.C., Structural insights on the role of antibodies in HIV-1 vaccine and therapy.
Cell. (2014). read more


Shingai, M., Donau, O.K., Plishka, R.J., Buckler-White, A., Mascola, J.R., Nabel, G.J., Nason, M.C., Montefiori, D., Moldt, B., Poignard, P., Diskin, R., Bjorkman, P.J., Eckhaus, M.A., Klein, F., Mouquet, H., Cetrulo Lorenzi, J.C., Gazumyan, A., Burton, D.R., Nussenzweig, M.C., Martin, M.A., and Nishimura, Y., Passive transfer of modest titers of potent and broadly neutralizing anti-HIV monoclonal antibodies block SHIV infection in macaques.
J Exp Med. (2014). read more


2013


Klein, F., Mouquet, H., Dosenovic, P., Scheid, J.F., Scharf, L., and Nussenzweig, M.C., Antibodies in HIV-1 vaccine development and therapy.
Science. (2013). read more


Shingai, M., Nishimura, Y., Klein, F., Mouquet, H., Donau, O.K., Plishka, R., Buckler-White, A., Seaman, M., Piatak, M., Jr., Lifson, J.D., Dimitrov, D.S., Nussenzweig, M.C., and Martin, M.A., Antibody-mediated immunotherapy of macaques chronically infected with SHIV suppresses viraemia.
Nature. (2013). read more


Klein, F., Diskin, R., Scheid, J.F., Gaebler, C., Mouquet, H., Georgiev, I.S., Pancera, M., Zhou, T., Incesu, R.B., Fu, B.Z., Gnanapragasam, P.N., Oliveira, T.Y., Seaman, M.S., Kwong, P.D., Bjorkman, P.J., and Nussenzweig, M.C., Somatic mutations of the immunoglobulin framework are generally required for broad and potent HIV-1 neutralization.
Cell. (2013). read more


Malbec, M., Porrot, F., Rua, R., Horwitz, J., Klein, F., Halper-Stromberg, A., Scheid, J.F., Eden, C., Mouquet, H., Nussenzweig, M.C., and Schwartz, O., Broadly neutralizing antibodies that inhibit HIV-1 cell to cell transmission.
J Exp Med. (2013). read more


West, A.P., Jr., Scharf, L., Horwitz, J., Klein, F., Nussenzweig, M.C., and Bjorkman, P.J., Computational analysis of anti-HIV-1 antibody neutralization panel data to identify potential functional epitope residues.
Proc Natl Acad Sci U S A. (2013). read more


Diskin, R., Klein, F., Horwitz, J.A., Halper-Stromberg, A., Sather, D.N., Marcovecchio, P.M., Lee, T., West, A.P., Jr., Gao, H., Seaman, M.S., Stamatatos, L., Nussenzweig, M.C., and Bjorkman, P.J., Restricting HIV-1 pathways for escape using rationally designed anti-HIV-1 antibodies.
J Exp Med. (2013). read more


Barouch, D.H., Whitney, J.B., Moldt, B., Klein, F., Oliveira, T.Y., Liu, J., Stephenson, K.E., Chang, H.W., Shekhar, K., Gupta, S., Nkolola, J.P., Seaman, M.S., Smith, K.M., Borducchi, E.N., Cabral, C., Smith, J.Y., Blackmore, S., Sanisetty, S., Perry, J.R., Beck, M., Lewis, M.G., Rinaldi, W., Chakraborty, A.K., Poignard, P., Nussenzweig, M.C., and Burton, D.R., Therapeutic efficacy of potent neutralizing HIV-1-specific monoclonal antibodies in SHIV-infected rhesus monkeys.
Nature. (2013). read more


Horwitz, J.A., Halper-Stromberg, A., Mouquet, H., Gitlin, A.D., Tretiakova, A., Eisenreich, T.R., Malbec, M., Gravemann, S., Billerbeck, E., Dorner, M., Buning, H., Schwartz, O., Knops, E., Kaiser, R., Seaman, M.S., Wilson, J.M., Rice, C.M., Ploss, A., Bjorkman, P.J., Klein, F., and Nussenzweig, M.C., HIV-1 suppression and durable control by combining single broadly neutralizing antibodies and antiretroviral drugs in humanized mice.
Proc Natl Acad Sci U S A. (2013). read more


Hoot, S., McGuire, A.T., Cohen, K.W., Strong, R.K., Hangartner, L., Klein, F., Diskin, R., Scheid, J.F., Sather, D.N., Burton, D.R., and Stamatatos, L., Recombinant HIV envelope proteins fail to engage germline versions of anti-CD4bs bNAbs.
PLoS Pathog. (2013). read more


Guermonprez, P., Helft, J., Claser, C., Deroubaix, S., Karanje, H., Gazumyan, A., Darasse-Jeze, G., Telerman, S.B., Breton, G., Schreiber, H.A., Frias-Staheli, N., Billerbeck, E., Dorner, M., Rice, C.M., Ploss, A., Klein, F., Swiecki, M., Colonna, M., Kamphorst, A.O., Meredith, M., Niec, R., Takacs, C., Mikhail, F., Hari, A., Bosque, D., Eisenreich, T., Merad, M., Shi, Y., Ginhoux, F., Renia, L., Urban, B.C., and Nussenzweig, M.C., Inflammatory Flt3l is essential to mobilize dendritic cells and for T cell responses during Plasmodium infection.
Nat Med. (2013). read more


Gaebler, C., Gruell, H., Velinzon, K., Scheid, J.F., Nussenzweig, M.C., and Klein, F., Isolation of HIV-1-reactive antibodies using cell surface-expressed gp160Deltac(BaL.).
J Immunol Methods. (2013). read more


2012


Pietzsch, J., Gruell, H., Bournazos, S., Donovan, B.M., Klein, F., Diskin, R., Seaman, M.S., Bjorkman, P.J., Ravetch, J.V., Ploss, A., and Nussenzweig, M.C., A mouse model for HIV-1 entry.
Proc Natl Acad Sci U S A. (2012). read more


Klein, F., Gaebler, C., Mouquet, H., Sather, D.N., Lehmann, C., Scheid, J.F., Kraft, Z., Liu, Y., Pietzsch, J., Hurley, A., Poignard, P., Feizi, T., Morris, L., Walker, B.D., Fatkenheuer, G., Seaman, M.S., Stamatatos, L., and Nussenzweig, M.C., Broad neutralization by a combination of antibodies recognizing the CD4 binding site and a new conformational epitope on the HIV-1 envelope protein.
J Exp Med. (2012). read more


Klein, F., Halper-Stromberg, A., Horwitz, J.A., Gruell, H., Scheid, J.F., Bournazos, S., Mouquet, H., Spatz, L.A., Diskin, R., Abadir, A., Zang, T., Dorner, M., Billerbeck, E., Labitt, R.N., Gaebler, C., Marcovecchio, P.M., Incesu, R.B., Eisenreich, T.R., Bieniasz, P.D., Seaman, M.S., Bjorkman, P.J., Ravetch, J.V., Ploss, A., and Nussenzweig, M.C., HIV therapy by a combination of broadly neutralizing antibodies in humanized mice.
Nature. (2012). read more


Vehreschild, J.J., Moritz, G., Vehreschild, M.J., Arenz, D., Mahne, M., Bredenfeld, H., Chemnitz, J., Klein, F., Cremer, B., Boll, B., Kaul, I., Wassmer, G., Hallek, M., Scheid, C., and Cornely, O.A., Efficacy and safety of moxifloxacin as antibacterial prophylaxis for patients receiving autologous haematopoietic stem cell transplantation: a randomised trial.
Int J Antimicrob Agents. (2012). read more


Sather, D.N., Carbonetti, S., Kehayia, J., Kraft, Z., Mikell, I., Scheid, J.F., Klein, F., and Stamatatos, L., Broadly neutralizing antibodies developed by an HIV-positive elite neutralizer exact a replication fitness cost on the contemporaneous virus.
J Virol. (2012). read more


2011


Scheid, J.F., Mouquet, H., Ueberheide, B., Diskin, R., Klein, F., Oliveira, T.Y., Pietzsch, J., Fenyo, D., Abadir, A., Velinzon, K., Hurley, A., Myung, S., Boulad, F., Poignard, P., Burton, D.R., Pereyra, F., Ho, D.D., Walker, B.D., Seaman, M.S., Bjorkman, P.J., Chait, B.T., and Nussenzweig, M.C., Sequence and structural convergence of broad and potent HIV antibodies that mimic CD4 binding.
Science. (2011). read more


Mouquet, H., Klein, F., Scheid, J.F., Warncke, M., Pietzsch, J., Oliveira, T.Y., Velinzon, K., Seaman, M.S., and Nussenzweig, M.C., Memory B cell antibodies to HIV-1 gp140 cloned from individuals infected with clade A and B viruses.
PLoS One. (2011). read more


Diskin, R., Scheid, J.F., Marcovecchio, P.M., West, A.P., Jr., Klein, F., Gao, H., Gnanapragasam, P.N., Abadir, A., Seaman, M.S., Nussenzweig, M.C., and Bjorkman, P.J., Increasing the potency and breadth of an HIV antibody by using structure-based rational design.
Science. (2011). read more


2010


Pietzsch, J., Scheid, J.F., Mouquet, H., Klein, F., Seaman, M.S., Jankovic, M., Corti, D., Lanzavecchia, A., and Nussenzweig, M.C., Human anti-HIV-neutralizing antibodies frequently target a conserved epitope essential for viral fitness.
J Exp Med. (2010). read more


2009


Klein, F. and Hallek, M., Does the removal of non-affected lymph nodes of a neoplastic focus weaken the immunologic defense?.
Dtsch Med Wochenschr. (2009). read more


2007


Feldhahn, N., Henke, N., Melchior, K., Duy, C., Soh, B.N., Klein, F., von Levetzow, G., Giebel, B., Li, A., Hofmann, W.K., Jumaa, H., and Muschen, M., Activation-induced cytidine deaminase acts as a mutator in BCR-ABL1-transformed acute lymphoblastic leukemia cells.
J Exp Med. (2007). read more


2006


Klein, F., Feldhahn, N., Herzog, S., Sprangers, M., Mooster, J.L., Jumaa, H., and Muschen, M., BCR-ABL1 induces aberrant splicing of IKAROS and lineage infidelity in pre-B lymphoblastic leukemia cells.
Oncogene. (2006). read more


2005


Feldhahn, N., Klein, F., Mooster, J.L., Hadweh, P., Sprangers, M., Wartenberg, M., Bekhite, M.M., Hofmann, W.K., Herzog, S., Jumaa, H., Rowley, J.D., and Muschen, M., Mimicry of a constitutively active pre-B cell receptor in acute lymphoblastic leukemia cells.
J Exp Med. (2005). read more


Feldhahn, N., Rio, P., Soh, B.N., Liedtke, S., Sprangers, M., Klein, F., Wernet, P., Jumaa, H., Hofmann, W.K., Hanenberg, H., Rowley, J.D., and Muschen, M., Deficiency of Bruton's tyrosine kinase in B cell precursor leukemia cells.
Proc Natl Acad Sci U S A. (2005). read more


Soh, B.N., Klein, F., Feldhahn, N., and Muschen, M., B-lymphoid or myeloid lineage identity of cell lines derived from chronic myeloid leukemia blast crisis.
Cancer Genet Cytogenet. (2005). read more


Klein, F., Feldhahn, N., Mooster, J.L., Sprangers, M., Hofmann, W.K., Wernet, P., Wartenberg, M., and Muschen, M., Tracing the pre-B to immature B cell transition in human leukemia cells reveals a coordinated sequence of primary and secondary IGK gene rearrangement, IGK deletion, and IGL gene rearrangement.
J Immunol. (2005). read more


Klein, F., Feldhahn, N., and Muschen, M., Interference of BCR-ABL1 kinase activity with antigen receptor signaling in B cell precursor leukemia cells.
Cell Cycle. (2004). read more


Klein, F., Feldhahn, N., Harder, L., Wang, H., Wartenberg, M., Hofmann, W.K., Wernet, P., Siebert, R., and Muschen, M., The BCR-ABL1 kinase bypasses selection for the expression of a pre-B cell receptor in pre-B acute lymphoblastic leukemia cells.
J Exp Med. (2004). read more


2003


Klein, F., Feldhahn, N., Lee, S., Wang, H., Ciuffi, F., von Elstermann, M., Toribio, M.L., Sauer, H., Wartenberg, M., Barath, V.S., Kronke, M., Wernet, P., Rowley, J.D., and Muschen, M., T lymphoid differentiation in human bone marrow.
Proc Natl Acad Sci U S A. (2003). read more


Wartenberg, M., Ling, F.C., Muschen, M., Klein, F., Acker, H., Gassmann, M., Petrat, K., Putz, V., Hescheler, J., and Sauer, H., Regulation of the multidrug resistance transporter P-glycoprotein in multicellular tumor spheroids by hypoxia-inducible factor (HIF-1) and reactive oxygen species.
FASEB J. (2003). read more